Author

Jack Ryan

Early Embryogenesis: Fertilization
Signaling: Morphogens and Gradients; Contact-Mediated Signaling; Secreted Signals
Ecological Developmental Biology: Developmental Symbiosis; Environ & Molecular Change
Organism: Zebrafish

Object Description

Ocular development involves intricate network of cells derived from forebrain neuroectoderm, surface ectoderm and neural crest-derived perioptic mesenchyme (POM). Retinoic acid (RA) synthesized by retinaldehyde dehydrogenases (Raldh1, Raldh2 and Raldh3) functions in a paracrine fashion to guide morphogenetic movements in retina and POM. Pitx2, encoding homeodomain transcription factor is a direct target of RA that regulates Dkk2 (an antagonist of Wnt/b-catenin signaling) in POM to locally suppress Wnt signaling to ensure proper eye development. Raldh double null mutant (R1:R3-\-) embryos exhibit severe ocular phenotype characterized by lack of upper eyelid, disoriented optic cup, corneal dysgenesis and microphthalmia. In situ hybridization with Dkk2 riboprobe on frontal sections of E12.5 wild-type (WT) and double null (R1:R3-/-) mice eyes shows reduced expression of Dkk2 in POM of R1:R3-/- compared to WT suggesting that activated Wnt signaling could lead to these ocular defects.

Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Aenean commodo ligula eget dolor. Aenean massa. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Donec quam felis, ultricies nec, pellentesque eu, pretium quis, sem. Nulla consequat massa quis enim. Donec pede justo, fringilla vel, aliquet nec, vulputate eget, arcu.

In enim justo, rhoncus ut, imperdiet a, venenatis vitae, justo. Nullam dictum felis eu pede mollis pretium. Integer tincidunt. Cras dapibus. Vivamus elementum semper nisi. Aenean vulputate eleifend tellus. Aenean leo ligula, porttitor eu, consequat vitae, eleifend ac, enim. Aliquam lorem ante, dapibus in, viverra quis, feugiat a, tellus. Phasellus viverra nulla ut metus varius laoreet. Quisque rutrum. Aenean imperdiet. Etiam ultricies nisi vel augue. Curabitur ullamcorper ultricies nisi. Nam eget dui. Etiam rhoncus. Maecenas tempus, tellus eget condimentum rhoncus, sem quam semper libero, sit amet adipiscing sem neque sed ipsum.

Comment on this Object

You must be logged in to CoRe to comment. Please login or create an account.